期刊
CELL REPORTS
卷 34, 期 7, 页码 -出版社
CELL PRESS
DOI: 10.1016/j.celrep.2021.108752
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资金
- NIH [G12 MD007591]
- NSF [DBI-1337513, DBI-2018408]
- Eunice Kennedy Shriver National Institute of Child Health and Human Development (NICHD) [R01 HD090007, P50 HD98593]
Spermatogonial stem cells maintain spermatogenesis by balancing self-renewal and differentiation, with mTORC1 signaling playing a crucial role in the process.
Spermatogonial stem cells (SSCs) sustain spermatogenesis by balancing self-renewal and initiation of differentiation to produce progenitor spermatogonia committed to forming sperm. To define the regulatory logic among SSCs and progenitors, we performed single-cell RNA velocity analyses and validated results in vivo. A predominant quiescent SSC population spawns a small subset of cell-cycle-activated SSCs via mitogen-activated protein kinase (MAPK)/AKT signaling. Activated SSCs form early progenitors and mTORC1 inhibition drives activated SSC accumulation consistent with blockade to progenitor formation. Mechanistically, mTORC1 inhibition suppresses transcription among spermatogonia and specifically alters expression of insulin growth factor (IGF) signaling in early progenitors. Tex14(-/-) testes lacking intercellular bridges do not accumulate activated SSCs following mTORC1 inhibition, indicating that steady-state mTORC1 signaling drives activated SSCs to produce progenitor clones. These results are consistent with a model of SSC self-renewal dependent on interconversion between activated and quiescent SSCs, and mTORC1-dependent initiation of differentiation from SSCs to progenitor clones.
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