4.7 Article

An Immunomodulatory Miniaturized 3D Screening Platform Using Liquefied Capsules

期刊

ADVANCED HEALTHCARE MATERIALS
卷 10, 期 10, 页码 -

出版社

WILEY
DOI: 10.1002/adhm.202001993

关键词

host' s immune response; immunomodulatory 3D platforms; macrophages; mesenchymal stem cells; polymers

资金

  1. Portuguese Foundation for Science and Technology (FCT) [SFRH/BD/130194/2017]
  2. project CIRCUS [PTDC/BTM-MAT/31064/2017]
  3. European Research Council [ERC-2014-AdG-669858]
  4. FCT/MEC [UIDB/50011/2020, UIDP/50011/2020]
  5. FEDER under the PT2020 Partnership Agreement
  6. Fundação para a Ciência e a Tecnologia [SFRH/BD/130194/2017] Funding Source: FCT

向作者/读者索取更多资源

The study proposes the use of liquefied capsules as an immunomodulatory 3D platform to evaluate the immune response following the implantation of biomedical devices. Experimental results show that chitosan-ending capsules and the presence of MSCs promote a more regenerative polarization balance of macrophages.
A critical determinant of successful clinical outcomes is the host's response to the biomaterial. Therefore, the prediction of the immunomodulatory bioperformance of biomedical devices following implantation is of utmost importance. Herein, liquefied capsules are proposed as immunomodulatory miniaturized 3D platforms for the high-content combinatorial screening of different polymers that could be used generically in scaffolds. Additionally, the confined and liquefied core of capsules affords a cell-mediated 3D assembly with bioinstructive microplatforms, allowing to study the potential synergistic effect that cells in tissue engineering therapies have on the immunological environment before implantation. As a proof-of-concept, three different polyelectrolytes, ranging in charge density and source, are used. Poly(L-lysine)-, alginate-, and chitosan-ending capsules with or without encapsulated mesenchymal stem/stromal cells (MSCs) are placed on top of a 2D culture of macrophages. Results show that chitosan-ending capsules, as well as the presence of MSCs, favor the balance of macrophage polarization toward a more regenerative profile, through the up-regulation of anti-inflammatory markers, and the release of pro-regenerative cytokines. Overall, the developed system enables the study of the immunomodulatory bioperformance of several polymers in a cost-effective and scalable fashion, while the paracrine signaling between encapsulated cells and the immunological environment can be simultaneously evaluated.

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