期刊
STEM CELL RESEARCH & THERAPY
卷 12, 期 1, 页码 -出版社
BMC
DOI: 10.1186/s13287-021-02203-1
关键词
ASCs; EPCs; ECM; Vascularization
资金
- Natural Science Foundation of China [81771351]
- support program for young backbone teachers in China Medical University [QGZD2018076]
The co-culture of ASCs/EPCs enhances gene expression related to skin and angiogenesis, and accelerates the repair of skin defects in vivo. This approach may offer a promising alternative to traditional single-seed cell therapy for vascularized skin regeneration.
BackgroundThe repair of large-scale full-thickness skin defects represents a challenging obstacle in skin tissue engineering. To address the most important problem in skin defect repair, namely insufficient blood supply, this study aimed to find a method that could promote the formation of vascularized skin tissue.MethodThe phenotypes of ASCs and EPCs were identified respectively, and ASCs/EPCs were co-cultured in vitro to detect the expression of dermal and angiogenic genes. Furthermore, the co-culture system combined with dermal extracellular matrix hydrogel was used to repair the full-scale skin defects in rats.ResultThe co-culture of ASCs/EPCs could increase skin- and angiogenesis-related gene expression in vitro. The results of in vivo animal experiments demonstrated that the ASCs/EPCs group could significantly accelerate the repair of skin defects by promoting the regeneration of vascularized skin.ConclusionIt is feasible to replace traditional single-seed cells with the ASC/EPC co-culture system for vascularized skin regeneration. This system could ultimately enable clinicians to better repair the full-thickness skin defects and avoid donor site morbidity.
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