Silicon nitride: a potent solid-state bioceramic inactivator of ssRNA viruses

Surface inactivation of human microbial pathogens has a long history. The Smith Papyrus (2600 similar to 2200 B.C.) described the use of copper surfaces to sterilize chest wounds and drinking water. Brass and bronze on doorknobs can discourage microbial spread in hospitals, and metal-base surface coatings are used in hygiene-sensitive environments, both as inactivators and modulators of cellular immunity. A limitation of these approaches is that the reactive oxygen radicals (ROS) generated at metal surfaces also damage human cells by oxidizing their proteins and lipids. Silicon nitride (Si3N4) is a non-oxide ceramic compound with known surface bacterial resistance. We show here that off-stoichiometric reactions at Si3N4 surfaces are also capable of inactivating different types of single-stranded RNA (ssRNA) viruses independent of whether their structure presents an envelop or not. The antiviral property of Si3N4 derives from a hydrolysis reaction at its surface and the subsequent formation of reactive nitrogen species (RNS) in doses that could be metabolized by mammalian cells but are lethal to pathogens. Real-time reverse transcription (RT)-polymerase chain reaction (PCR) tests of viral RNA and in situ Raman spectroscopy suggested that the products of Si3N4 hydrolysis directly react with viral proteins and RNA. Si3N4 may have a role in controlling human epidemics related to ssRNA mutant viruses.

Automated summary

Surface inactivation of human microbial pathogens has a long history, with the use of copper surfaces and metal coatings being common methods. However, Si3N4 has emerged as a potential material for combating ssRNA viruses due to its hydrolysis reaction and generation of reactive nitrogen species. This non-oxide ceramic compound may play a role in controlling human epidemics related to ssRNA mutant viruses.