期刊
SCIENTIFIC REPORTS
卷 11, 期 1, 页码 -出版社
NATURE RESEARCH
DOI: 10.1038/s41598-021-85129-1
关键词
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资金
- NIGMS [5R01GM120033]
- U.S. Army Medical Research [DAMD170110754]
- Cynthia and Antony Petrello Endowment
- National Institute of Diabetes and Digestive and Kidney Diseases [T32DK07521-16]
- MINECO [SAF-2015-70866R]
- FPI MICINN predoctoral Fellowship
- Proteomics Center at Stony Brook University [NIH/NCRR 1S10 RR023680]
- BCM IDDRC Grant from the Eunice Kennedy Shriver National Institute of Child Health and Human Development [P50HD10355]
- Mark A. Wallace Endowment
- U.S. Department of Defense (DOD) [DAMD170110754] Funding Source: U.S. Department of Defense (DOD)
This study identified the epitope for the NSC-6 antibody as the signaling protein BASP1, which plays a key role in neurite outgrowth and plasticity. Western blot analysis revealed multiple BASP1 isoforms with varying expression levels, correlating with distinct developmental stages in mouse and human brains. The NSC-6 antibody could be a useful marker for neural stem cells in various developmental stages.
The mechanisms responsible for determining neural stem cell fate are numerous and complex. To begin to identify the specific components involved in these processes, we generated several mouse neural stem cell (NSC) antibodies against cultured mouse embryonic neurospheres. Our immunohistochemical data showed that the NSC-6 antibody recognized NSCs in the developing and postnatal murine brains as well as in human brain organoids. Mass spectrometry revealed the identity of the NSC-6 epitope as brain abundant, membrane-attached signal protein 1 (BASP1), a signaling protein that plays a key role in neurite outgrowth and plasticity. Western blot analysis using the NSC-6 antibody demonstrated multiple BASP1 isoforms with varying degrees of expression and correlating with distinct developmental stages. Herein, we describe the expression of BASP1 in NSCs in the developing and postnatal mammalian brains and human brain organoids, and demonstrate that the NSC-6 antibody may be a useful marker of these cells.
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