Graphene oxide assisted light-up aptamer selection against Thioflavin T for label-free detection of microRNA

We selected an aptamer against a fluorogenic dye called Thioflavin T (ThT). Aptamers are single-stranded DNA that can bind a specific target. We selected the ThT aptamer using graphene oxide assisted SELEX and a low-cost Open qPCR instrument. We optimized, minimized, and characterized the best aptamer candidate against ThT. The aptamer, ThT dye, and the enzymatic strand displacement amplification (SDA) were used in a label-free approach to detect the micro RNA miR-215 in saliva and serum. The aptamer confers higher specificity than intercalating dyes but without expensive covalently modified DNA probes. This isothermal, low-cost, simple method can detect both DNA and RNA. The target, miR-215, was detected with a limit of detection of 2.6 nM.

Automated summary

Researchers developed a low-cost, simple, and label-free method with high specificity for detecting DNA and RNA simultaneously by selecting an aptamer against the fluorogenic dye Thioflavin T (ThT), optimizing and minimizing it, and characterizing its interactions. This approach successfully detected the target miR-215 in saliva and serum, showing a detection limit of 2.6 nM.