期刊
SCIENTIFIC REPORTS
卷 11, 期 1, 页码 -出版社
NATURE RESEARCH
DOI: 10.1038/s41598-021-83640-z
关键词
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资金
- NIH COBRE grant [P30GM103324]
Researchers developed a low-cost, simple, and label-free method with high specificity for detecting DNA and RNA simultaneously by selecting an aptamer against the fluorogenic dye Thioflavin T (ThT), optimizing and minimizing it, and characterizing its interactions. This approach successfully detected the target miR-215 in saliva and serum, showing a detection limit of 2.6 nM.
We selected an aptamer against a fluorogenic dye called Thioflavin T (ThT). Aptamers are single-stranded DNA that can bind a specific target. We selected the ThT aptamer using graphene oxide assisted SELEX and a low-cost Open qPCR instrument. We optimized, minimized, and characterized the best aptamer candidate against ThT. The aptamer, ThT dye, and the enzymatic strand displacement amplification (SDA) were used in a label-free approach to detect the micro RNA miR-215 in saliva and serum. The aptamer confers higher specificity than intercalating dyes but without expensive covalently modified DNA probes. This isothermal, low-cost, simple method can detect both DNA and RNA. The target, miR-215, was detected with a limit of detection of 2.6 nM.
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