4.7 Article

Helicobacter pylori type 4 secretion systems as gastroduodenal disease markers

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SCIENTIFIC REPORTS
卷 11, 期 1, 页码 -

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NATURE RESEARCH
DOI: 10.1038/s41598-021-83862-1

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资金

  1. Ministry of Education, Culture, Sports, Science, and Technology (MEXT) of Japan [26640114, 15H02657, 16H05191]
  2. Special Coordination Funds for Promoting Science and Technology from the MEXT of Japan
  3. National Institutes of Health [DK62813]
  4. Okinawa Prefectural Government
  5. Japanese Government (Monbukagakusho: MEXT)
  6. Grants-in-Aid for Scientific Research [15H02657, 26640114] Funding Source: KAKEN

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The acquisition of tfs3/4 ICE was common in H. pylori strains in patients with gastroduodenal disease in Vietnam, and the complete cluster of tfs3 ICE was a reliable marker for the severity of disease in the H. pylori infected population.
Although the type 4 secretion system of the integrating and conjugative elements (tfs ICE) is common in Helicobacter pylori, its clinical association with the cag pathogenicity island (cagPAI) have not yet been well-investigated. In this study, Vietnamese patient H. pylori samples (46 duodenal ulcer (DU), 51 non-cardia gastric cancer (NCGC), 39 chronic gastritis (CG)) were fully sequenced using next-generation sequencing and assembled into contigs. tfs3, tfs4, and cagPAI genes were compared with the public database. Most (94%) H. pylori strains possessed a complete cagPAI, which was the greatest risk factor for clinical outcomes, while the prevalences of tfs3 and tfs4 were 45% and 77%, respectively. Complete tfs3 and tfs4 were found in 18.3% and 17.6% of strains, respectively. The prevalence of H. pylori strains with complete tfs3 ICE in DU patients was significantly higher than that in NCGC patients (30.4% vs 11.7%, P<0.05). In addition, the prevalence of strains with complete tfs3 ICE and cagPAI was significantly higher in DU patients than that in NCGC (28.4% vs 9.8%, P=0.038) and CG patients (28.2% vs 7.7%, P=0.024). cagPAI and complete tfs3 increased the risk of DU compared to NCGC (OR=3.56, 95%CI: 1.1-14.1, P=0.038) and CG (OR=4.64, 95%CI: 1.1-27.6, P=0.024). A complete cluster of tfs3 ICE was associated with gastroduodenal diseases in Vietnam. However, there was a low prevalence of the dupA/complete dupA cluster (15.4%) in the Vietnam strains. The prevalence of cagPAI in Vietnam strains was significantly higher than in US (P=0.01) and Indonesia (P<0.0001); the prevalence of the dupA cluster was also higher in the Vietnam strains than in the Indonesian strains (P<0.05). In addition, the prevalence of ctkA, an accessory gene of tfs3, was significantly different between Vietnam and US strains (28% vs 2%, P=0.0002). In summary, the acquisition of tfs3/4 ICE was common in H. pylori strains in patients with gastroduodenal disease in Vietnam, and the complete cluster of tfs3 ICE was a reliable marker for the severity of disease in the H. pylori infected population.

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