Culture and differentiation of rabbit intestinal organoids and organoid-derived cell monolayers

Organoids emulate many aspects of their parental tissue and are therefore used to study pathogen-host interactions and other complex biological processes. Here, we report a robust protocol for the isolation, maintenance and differentiation of rabbit small intestinal organoids and organoid-derived cell monolayers. Our rabbit intestinal spheroid and monolayer cultures grew most efficiently in L-WRN-conditioned medium that contained Wnt, R-spondin and Noggin, and that had been supplemented with ROCK and TGF-beta inhibitors. Organoid and monolayer differentiation was initiated by reducing the concentration of the L-WRN-conditioned medium and by adding ROCK and Notch signalling inhibitors. Immunofluorescence staining and RT-qPCR demonstrated that our organoids contained enterocytes, enteroendocrine cells, goblet cells and Paneth cells. Finally, we infected rabbit organoids with Rabbit calicivirus Australia-1, an enterotropic lagovirus that-like many other caliciviruses-does not grow in conventional cell culture. Despite testing various conditions for inoculation, we did not detect any evidence of virus replication, suggesting either that our organoids do not contain suitable host cell types or that additional co-factors are required for a productive infection of rabbit organoids with Rabbit calicivirus Australia-1.

Automated summary

This study introduced a robust protocol for isolating, maintaining, and differentiating rabbit small intestinal organoids and organoid-derived cell monolayers. The organoids grew most efficiently in L-WRN-conditioned medium supplemented with specific inhibitors, showing differentiation into various cell types. Despite efforts to infect the rabbit organoids with Rabbit calicivirus Australia-1, no evidence of virus replication was detected, suggesting potential requirements for specific host cell types or additional co-factors in productive infection.