4.7 Article

High β-Glucan Barley Supplementation Improves Glucose Tolerance by Increasing GLP-1 Secretion in Diet-Induced Obesity Mice

期刊

NUTRIENTS
卷 13, 期 2, 页码 -

出版社

MDPI
DOI: 10.3390/nu13020527

关键词

barley; β -glucan; L cell; glucagon-like peptide 1 (GLP-1); glucose tolerance

资金

  1. Council of Japan Barley Foods Promotion

向作者/读者索取更多资源

Intake of high beta-glucan barley (HGB) improved glucose tolerance by increasing the levels of short-chain fatty acids (SCFAs) and glucagon-like peptide-1 (GLP-1) in the cecum, along with a rise in L cell number and subsequent GLP-1 secretion.
The aim of this study was to investigate the underlying mechanism for the improvement of glucose tolerance following intake of high beta-glucan barley (HGB) in terms of intestinal metabolism. C57BL/6J male mice were fed a fatty diet supplemented with HGB corresponding to 5% of dietary fiber for 83 days. An oral glucose tolerance test was performed at the end of the experimental period. The concentration of short-chain fatty acids (SCFAs) in the cecum was analyzed by GC-MS (gas chromatography-mass spectrometry). The mRNA expression levels related to L cell function in the ileum were measured by real-time PCR. Glucagon-like peptide-1 (GLP-1) levels in the portal vein and cecal content were assessed by enzyme-linked immunosorbent assay. GLP-1-producing L cells of the ileum were quantified by immunohistochemistry. HGB intake improved glucose tolerance and increased the cecal levels of SCFAs, acetate, and propionate. The number of GLP-1-positive L cells in the HGB group was significantly higher than in the control group. GLP-1 levels in the portal vein and cecal GLP-1 pool size in the HGB group were significantly higher than the control group. In conclusion, we report improved glucose tolerance after HGB intake induced by an increase in L cell number and subsequent rise in GLP-1 secretion.

作者

我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。

评论

主要评分

4.7
评分不足

次要评分

新颖性
-
重要性
-
科学严谨性
-
评价这篇论文

推荐

暂无数据
暂无数据