期刊
NUTRIENTS
卷 13, 期 3, 页码 -出版社
MDPI
DOI: 10.3390/nu13030742
关键词
free fatty acids; butyric acid; GC– MS method; celiac disease; colorectal cancer
资金
- Foundation Ente Cassa di Risparmio di Fi renze
- Italian Society for Celiac Disease and Foundation for Celicac Disease [007_FC_2016]
- regional contribution of The Programma Attuativo Regionale (Toscana) - FSC [4042.16092014.066000029]
Changes in circulating levels of free fatty acids, including short chain, medium chain, and long chain fatty acids, are associated with metabolic, gastrointestinal, and malignant diseases. Analysis of FFA profiles in patients with celiac disease, adenomatous polyposis, and colorectal cancer compared to healthy controls revealed distinct differences, with butyric acid potentially serving as a biomarker for celiac disease screening. This study suggests gas chromatography-mass spectrometry as a suitable method for analyzing FFA composition in various gastrointestinal diseases.
Altered circulating levels of free fatty acids (FFAs), namely short chain fatty acids (SCFAs), medium chain fatty acids (MCFAs), and long chain fatty acids (LCFAs), are associated with metabolic, gastrointestinal, and malignant diseases. Hence, we compared the serum FFA profile of patients with celiac disease (CD), adenomatous polyposis (AP), and colorectal cancer (CRC) to healthy controls (HC). We enrolled 44 patients (19 CRC, 9 AP, 16 CD) and 16 HC. We performed a quantitative FFA evaluation with the gas chromatography-mass spectrometry method (GC-MS), and we performed Dirichlet-multinomial regression in order to highlight disease-specific FFA signature. HC showed a different composition of FFAs than CRC, AP, and CD patients. Furthermore, the partial least squares discriminant analysis (PLS-DA) confirmed perfect overlap between the CRC and AP patients and separation of HC from the diseased groups. The Dirichlet-multinomial regression identified only strong positive association between CD and butyric acid. Moreover, CD patients showed significant interactions with age, BMI, and gender. In addition, among patients with the same age and BMI, being male compared to being female implies a decrease of the CD effect on the (log) prevalence of butyric acid in FFA composition. Our data support GC-MS as a suitable method for the concurrent analysis of circulating SCFAs, MCFAs, and LCFAs in different gastrointestinal diseases. Furthermore, and notably, we suggest for the first time that butyric acid could represent a potential biomarker for CD screening.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据