期刊
ENVIRONMENTAL MICROBIOLOGY
卷 18, 期 8, 页码 2743-2753出版社
WILEY
DOI: 10.1111/1462-2920.13345
关键词
-
类别
资金
- Natural Environment Research Council (NERC) [NE/J009725/1, NE/J009555/1]
- University of Warwick Postgraduate Research Scholarship
- NERC
- Earth and Life Systems Alliance (ELSA) at the University of East Anglia
- Natural Environment Research Council [NE/J009725/1, NE/J009555/1] Funding Source: researchfish
- NERC [NE/J009555/1, NE/J009725/1] Funding Source: UKRI
Emissions of biogenic volatile organic compounds (bVOCs), are an important element in the global carbon cycle, accounting for a significant proportion of fixed carbon. They contribute directly and indirectly to global warming and climate change and have a major effect on atmospheric chemistry. Plants emit isoprene to the atmosphere in similar quantities to emissions of methane from all sources and each accounts for approximately one third of total VOCs. Although methanotrophs, capable of growth on methane, have been intensively studied, we know little of isoprene biodegradation. Here, we report the isolation of two isoprene-degrading strains from the terrestrial environment and describe the design and testing of polymerase chain reaction (PCR) primers targeting isoA, the gene encoding the active-site component of the conserved isoprene monooxygenase, which are capable of retrieving isoA sequences from isoprene-enriched environmental samples. Stable isotope probing experiments, using biosynthesized C-13-labelled isoprene, identified the active isoprene-degrading bacteria in soil. This study identifies novel isoprene-degrading strains using both culture-dependent and, for the first time, culture-independent methods and provides the tools and foundations for continued investigation of the biogeography and molecular ecology of isoprene-degrading bacteria.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据