multimodal visualization of the human retinal pigment epithelial mosaic

In vivo imaging of human retinal pigment epithelial (RPE) cells has been demonstrated through multiple adaptive optics (AO)-based modalities. However, whether consistent and complete information regarding the cellular structure of the RPE mosaic is obtained across these modalities remains uncertain due to limited comparisons performed in the same eye. Here, an imaging platform combining multimodal AO-scanning light ophthalmoscopy (AO-SLO) with AO-optical coherence tomography (AO-OCT) is developed to make a side-by-side comparison of the same RPE cells imaged across four modalities: AO-darkfield, AO-enhanced indocyanine green (AO-ICG), AO-infrared autofluorescence (AO-IRAF), and AO-OCT. Co-registered images were acquired in five subjects, including one patient with choroideremia. Multimodal imaging provided multiple perspectives of the RPE mosaic that were used to explore variations in RPE cell contrast in a subject-, location-, and even cell-dependent manner. Estimated cell-to-cell spacing and density were found to be consistent both across modalities and with normative data. Multimodal images from a patient with choroideremia illustrate the benefit of using multiple modalities to infer the cellular structure of the RPE mosaic in an affected eye, in which disruptions to the RPE mosaic may locally alter the signal strength, visibility of individual RPE cells, or even source of contrast in unpredictable ways. (c) 2021 Optical Society of America under the terms of the OSA Open Access Publishing Agreement

Automated summary

The study developed an imaging platform combining multimodal AO-SLO with AO-OCT to compare four different modalities for imaging RPE cells in the same eye. Consistent cellular structure information was obtained across modalities, with variations in RPE cell contrast explored in a subject-, location-, and even cell-dependent manner. The benefit of using multiple modalities to infer the cellular structure of the RPE mosaic in an affected eye was illustrated through multimodal imaging in a patient with choroideremia.