4.4 Article

In vitro biological activity of Salvia fruticosa Mill. infusion against amyloid β-peptide-induced toxicity and inhibition of GSK-3β, CK-1δ, and BACE-1 enzymes relevant to Alzheimer's disease

期刊

SAUDI PHARMACEUTICAL JOURNAL
卷 29, 期 3, 页码 236-243

出版社

ELSEVIER
DOI: 10.1016/j.jsps.2021.01.007

关键词

Lamiaceae; Neurotoxicity; Molecular modeling; Neuroprotection; Rosmarinic acid

资金

  1. Research Foundation of Erciyes University [TCD20124006, FCD-2018-7834]

向作者/读者索取更多资源

Salvia fruticosa infusion demonstrated neuroprotective effects against Alzheimer's disease-related neurotoxicity, potentially through regulation of p-GSK-3 beta protein.
Salvia species have been traditionally used to improve cognition and have been proved to be a potential natural treatment for Alzheimer's disease. Salvia fruticosa Mill. (Turkish sage or Greek sage) demonstrated to have anticholinergic effects in vitro. The aim of this study was to understand the mechanism underlying the neuroprotective effects of S. fruticosa infusion and its representative compound rosmarinic acid, which was detected by LC-DAD-ESI-MS/MS. The protective effects of the S. fruticosa infusion (SFINF) and its major substance rosmarinic acid (RA) on amyloid beta 1-42-induced cytotoxicity on SH-SY5Y cells together with p-GSK-3 beta activation were investigated. Their in vitro inhibitory effects against glycogen synthase kinase 3 beta, beta-secretase, and casein kinase 1 delta enzymes were also evaluated. The results showed that treatment with the all tested concentrations, SFINF significantly decreased A beta 1-42-induced cytotoxicity and exhibited promising in vitro glycogen synthase kinase 3 beta inhibitory activity below 10 mu g/mL (IC50 6.52 +/- 1.14 mu g/mL), in addition to beta-secretase inhibition (IC50 86 +/- 2.9 mu g/mL) and casein kinase 1 delta inhibition (IC50 121.57 +/- 4.00). The SFINF (100 mu g/mL and 250 mu g/mL) also activated the expression of p-GSK-3 beta in amyloid beta 1-42 treated SH-SY5Y cells. The outcomes of this study demonstrated that the S. fruticosa infusion possessed activity to prevent amyloid beta 1-42-induced neurotoxicity and provided proof that its mechanism may involve regulation of p-GSK-3 beta protein. (C) 2021 The Authors. Published by Elsevier B.V. on behalf of King Saud University.

作者

我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。

评论

主要评分

4.4
评分不足

次要评分

新颖性
-
重要性
-
科学严谨性
-
评价这篇论文

推荐

暂无数据
暂无数据