期刊
CELL DEATH & DISEASE
卷 12, 期 2, 页码 -出版社
SPRINGERNATURE
DOI: 10.1038/s41419-021-03462-9
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资金
- National Key Research and Development Program of China [2019YFF0301600]
- National Natural Science Foundation of China [31871146, 81800360, 81670253, 31771265, 32071169, 31971059]
The study demonstrates that acute glucose challenge induces vascular smooth muscle contraction through mitochondrial hyperpolarization-inactivated myosin phosphatase, representing a novel mechanism for vascular constriction and circulating metabolite clearance. Myosin phosphatase target subunit 1 plays a key role in mediating the activity of myosin phosphatase regulated by mitochondrial membrane potential.
It is well-established that long-term exposure of the vasculature to metabolic disturbances leads to abnormal vascular tone, while the physiological regulation of vascular tone upon acute metabolic challenge remains unknown. Here, we found that acute glucose challenge induced transient increases in blood pressure and vascular constriction in humans and mice. Ex vivo study in isolated thoracic aortas from mice showed that glucose-induced vascular constriction is dependent on glucose oxidation in vascular smooth muscle cells. Specifically, mitochondrial membrane potential (Delta Psi m), an essential component in glucose oxidation, was increased along with glucose influx and positively regulated vascular smooth muscle tone. Mechanistically, mitochondrial hyperpolarization inhibited the activity of myosin light chain phosphatase (MLCP) in a Ca2+-independent manner through activation of Rho-associated kinase, leading to cell contraction. However, Delta Psi m regulated smooth muscle tone independently of the small G protein RhoA, a major regulator of Rho-associated kinase signaling. Furthermore, myosin phosphatase target subunit 1 (MYPT1) was found to be a key molecule in mediating MLCP activity regulated by Delta Psi m. Delta Psi m positively phosphorylated MYPT1, and either knockdown or knockout of MYPT1 abolished the effects of glucose in stimulating smooth muscle contraction. In addition, smooth muscle-specific Mypt1 knockout mice displayed blunted response to glucose challenge in blood pressure and vascular constriction and impaired clearance rate of circulating metabolites. These results suggested that glucose influx stimulates vascular smooth muscle contraction via mitochondrial hyperpolarization-inactivated myosin phosphatase, which represents a novel mechanism underlying vascular constriction and circulating metabolite clearance.
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