Determination and validation of aprepitant in rat plasma using LC-MS/MS

Aim: The assessment of efficacy should be paralleled with extensive pharmacokinetic parameters, and a valid bioanalytical method is a pre-condition for accurate plasma concentration. Materials & methods: A simple, specific, rapid and sensitive LC-MS/MS method has been developed for quantitative analysis of aprepitant in rat plasma. A C18 column was used as stationary phase and the mobile phase consisted of a mixture of formic acid in water and formic acid in acetonitrile. Quantification was performed using multiple reaction monitoring mode. Results: The selectivity, linearity, accuracy, precision, robustness and ruggedness of the method were evaluated in accordance with bioanalytical method validation guideline of ICH and all results were within the acceptable range. Conclusion: The validated LC-MS/MS method was found to be useful for the quantitative analysis of aprepitant in rat plasma samples.

Automated summary

The study developed a LC-MS/MS method for quantitative analysis of aprepitant in rat plasma, and evaluated the method's selectivity, linearity, accuracy, precision, robustness, and ruggedness according to ICH guidelines, showing that the method is accurate and reliable for the analysis of aprepitant in rat plasma samples.