期刊
BIOANALYSIS
卷 13, 期 3, 页码 147-159出版社
Newlands Press Ltd
DOI: 10.4155/bio-2020-0297
关键词
affinity capture; hybrid LC– MS; ignotum peptide; LC– MS; monoclonal antibodies; quantification; quantitation; surrogate mAbs
The study evaluated the application of generic immunoaffinity reagents in measuring surrogate mAbs, and found that anti-mouse IgG1 was effective in quantifying mouse IgG1 mAbs in mouse serum. Peptides of unknown sequence were used for multiplex LC-MS quantification.
Background: Surrogate monoclonal antibodies (mAbs) used in preclinical in vivo studies can be challenging to quantify due to lack of suitable immunoaffinity reagents or unavailability of the mAb protein sequence. Generic immunoaffinity reagents were evaluated to develop sensitive LC-MS/MS assays. Peptides of unknown sequence can be used for selective LC-MS quantification. Results: anti-mouse IgG1 was found to be an effective immunoaffinity reagent, enabling quantification of mouse IgG1 mAbs in mouse serum. Selective peptides of unknown sequence were applied for multiplex LC-MS quantification of two rat mAbs co-dosed in mouse. Conclusion: Generic anti-mouse IgG subtype-specific antibodies can be used to improve assay sensitivity and peptides of unknown sequence can be used to quantify surrogate mAbs when the mAb protein sequence in unavailable.
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