Genome-Wide Association Study Dissects Resistance Loci against Bacterial Blight in a Diverse Rice Panel from the 3000 Rice Genomes Project

Background Bacterial blight (BB), caused by Xanthomonas oryzae pv. oryzae (Xoo) is one of the most devastating bacterial diseases of rice in temperate and tropical regions. Breeding and deployment of resistant cultivars carrying major resistance (R) genes has been the most effective approach for BB management. However, because of specific interaction of each R gene with the product of the corresponding pathogen avirulence or effector gene, new pathogen strains that can overcome the deployed resistance often emerge rapidly. To deal with ever-evolving Xoo, it is necessary to identify novel R genes and resistance quantitative trait loci (QTL). Results BB resistance of a diverse panel of 340 accessions from the 3000 Rice Genomes Project (3 K RGP) was evaluated by artificial inoculation with four representative Xoo strains, namely Z173 (C4), GD1358 (C5), V from China and PXO339 (P9a) from Philippines. Using the 3 K RG 4.8mio filtered SNP Dataset, a total of 11 QTL associated with BB resistance on chromosomes 4, 5, 11 and 12 were identified through a genome-wide association study (GWAS). Among them, eight resistance loci, which were narrowed down to relatively small genomic intervals, coincided with previously reported QTL or R genes, e.g. xa5, xa25, xa44(t). The other three QTL were putative novel loci associated with BB resistance. Linear regression analysis showed a dependence of BB lesion length on the number of favorable alleles, suggesting that pyramiding QTL using marker-assisted selection would be an effective approach for improving resistance. In addition, the Hap2 allele of LOC_Os11g46250 underlying qC5-11.1 was validated as positively regulating resistance against strain C5. Conclusions Our findings provide valuable information for the genetic improvement of BB resistance and application of germplasm resources in rice breeding programs.

Automated summary

Bacterial blight (BB) caused by Xanthomonas oryzae pv. oryzae (Xoo), is a devastating bacterial disease of rice in temperate and tropical regions. Breeding and deployment of resistant cultivars carrying major resistance (R) genes has been effective for BB management, but new strains that can overcome resistance emerge rapidly. This study identified 11 QTL associated with BB resistance, including 3 putative novel loci, through a genome-wide association study (GWAS) using a diverse panel of 340 accessions from the 3000 Rice Genomes Project. Linear regression analysis showed a dependence of BB lesion length on the number of favorable alleles, suggesting pyramiding QTL using marker-assisted selection would be an effective approach for improving resistance.