期刊
ZEBRAFISH
卷 18, 期 1, 页码 66-72出版社
MARY ANN LIEBERT, INC
DOI: 10.1089/zeb.2020.1966
关键词
mitochondrial sequence; ribosomal DNA; C-banding; chromosomal rearrangements; fish cytogenetics
资金
- CAPES (Coordenadoria de Aperfeicoamento de Ensino Superior)
- Fundacao Araucaria (Fundacao Araucaria de Apoio e Desenvolvimento Cientifico e Tecnologico do Estado do Parana)
- CNPq (Conselho Nacional de Desenvolvimento Cientifico e Tecnologico)
This study investigated the cytogenetic and molecular data of two Farlowella hahni populations, revealing chromosomal rearrangements and differences in rDNA sites. Fluorescence in situ hybridization showed variation in 18S rDNA and 5S rDNA sites between populations, while mitochondrial sequence analyses were unable to differentiate the two populations.
Farlowella is the second richest genus in Loricariinae, broadly distributed in freshwater streams and rivers of South America. In this article, we aimed to expand on the cytogenetic and molecular data available for two allopatric populations of Farlowella hahni. Both populations had diploid chromosome number 58, but with karyotype differences, indicative of chromosomal rearrangements. C-banding showed large heterochromatic blocks at telomeric regions in acrocentric chromosomes in both populations. Fluorescence in situ hybridization (FISH) revealed a single 18S rDNA site in both populations and a single 5S rDNA site for individuals from lower Parana River basin (native region) and multiple 5S rDNA sites for individuals from upper Parana River basin (non-native region). Mitochondrial sequence analyses did not separate the two F. hahni populations. The cytogenetic and molecular data obtained are relevant in a preliminary study and suggested the existence of cryptic diversity and the hypothesis that at least two Farlowella lineages may coexist in the Parana basin.
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