期刊
ZEBRAFISH
卷 18, 期 2, 页码 139-148出版社
MARY ANN LIEBERT, INC
DOI: 10.1089/zeb.2020.1950
关键词
laboratory animal; metacercaria; parasite; pet store; yellow grub
资金
- Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq) [309634/2018-0, 313674/2019-1]
- Companhia Riograndense de Saneamento (CORSAN) [006/16-DTEC]
This study focused on the infection by Clinostomum sp. in zebrafish from unknown origins, revealing swimming alterations and superficial yellow dots associated with muscular cysts, emphasizing the importance of using specific pathogen-free commercial creations or laboratory-reared zebrafish for research.
Many scientific studies still use zebrafish from pet stores as animal models, even cutting-edge researches. However, these animals differ genotypically and phenotypically between them. The importance of the use of standardized models is widely recognized. Besides that, another consequence of using zebrafish from unknown origins is the acquisition of parasitized animals. This study aimed to relate the infection by Clinostomum sp. in zebrafish. Animals sold as high standard were acquired from a commercial company. Swimming alterations and superficial yellow dots were observed in five zebrafish with clinical signs, which were isolated, euthanized, and necropsied. Muscular yellow cysts with metacercaria associated with lesions were observed. The muscular cysts were responsible for the superficial yellow dots as well as the swimming alterations. The prevalence was 2.5%, and the mean infection intensity was 7 digeneans/host. The cysts measured a mean of 1251.43 mu m long x 784.28 mu m wide. Metacercariae measured a mean of 4847 mu m long x 1353 mu m wide. This first report about infection by Clinostomum sp. in zebrafish is globally relevant since the host and the parasite genus currently overlap worldwide. Furthermore, this study sheds light on the importance of the specific pathogen-free commercial creations or laboratory-reared zebrafish for research.
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