期刊
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
卷 37, 期 3, 页码 -出版社
SPRINGER
DOI: 10.1007/s11274-021-03011-8
关键词
Membrane integrity; Monoterpenoids; Reactive oxygen species; YCA1
资金
- National Council for Scientific and Technological Development-CNPq [431538/2016-6, 304906/2017-4]
- Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES) [001]
- Cytogene Diagnosticos Moleculares Ltda
The mode of action of citral and geraniol on yeast cells involves disrupting the cell membrane and cell wall, leading to apoptosis and necrosis. Additionally, accumulation of intracellular ROS induces apoptosis mediated by metacaspase, resulting in cell death. Yeast cells with a deleted yca1 gene show high tolerance to citral and geraniol.
Essential oils and their main components, monoterpenes, have been proven to be important alternatives for the control of pathogenic and spoiling microorganisms, but the mode of action of these compounds is poorly understood. This work aimed to determine the mode of action of citral and geraniol on the model yeast Saccharomyces cerevisiae using a flow cytometry approach. Exponentially growing yeast cells were treated with different concentrations of citral and geraniol for 3 h, and evaluated for cell wall susceptibility to glucanase, membrane integrity, reactive oxygen species (ROS) accumulation, mitochondrial membrane potential, and metacaspase activity. Results provide strong evidence that citral and geraniol acute fungicidal activity against Saccharomyces cells involves the loss of membrane and cell wall integrity resulting in a dose-dependent apoptotic/necrotic cell death. However, yeast cells that escape this first cell membrane disruption, particularly evident on sub-lethal concentration, die by metacaspase-mediated apoptosis induced by the accumulation of intracellular ROS. The deleted mutant on the yca1 gene showed high tolerance to citral and geraniol.
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