期刊
WATER SCIENCE AND TECHNOLOGY
卷 83, 期 6, 页码 1399-1406出版社
IWA PUBLISHING
DOI: 10.2166/wst.2021.072
关键词
enzyme; fecal pollution; fluctuation; fluorescence; Sapporo; wastewater
资金
- JST-Mirai Program [JPMJMI18DB]
- JSPS KAKENHI [20KK0090, 19K21979, 17K18894]
- Gesuido Academic Incubation to Advanced (GAIA) Project of the Ministry of Land, Infrastructure, Transport and Tourism (MLIT) [2016-4]
- Northern Advancement Center for Science & Technology (NOASTEC) [2016-Startup-7]
- Toda Scholarship Foundation [2016-1]
- Grants-in-Aid for Scientific Research [20KK0090, 19K21979, 17K18894] Funding Source: KAKEN
A novel and simple method was developed to monitor Escherichia coli concentrations in river water, which was found to be reliable and applicable for estimating E. coli concentrations through monitoring GUS activities. The method is simple, rapid, reliable, inexpensive, and high throughput, making it useful for monitoring E. coli in river water.
Monitoring of Escherichia coli concentrations in river water (RW) is essential to identify fecal pollution of the river. The objective of this study was to assess the suitability of a novel, simple and high throughput method developed in our laboratory to enumerate E. coli concentrations in RW samples. The method is based on the use of the synthetic substrate specific for the beta-d-glucuronidase (GUS) produced by E. coli. GUS activities and E. coli concentrations were monitored at eight selected sites in rivers running through Sapporo, Japan. Because the fluorescence intensities of the synthetic substrate in the RW samples increased linearly over a 4-h incubation period, we could estimate the GUS activities of the RW samples. The GUS activities were highly correlated with E. coli concentrations at >100 most probable numbers 100 mL(-1) with a correlation coefficient of 0.87. The GUS activities of the RW samples collected from all sampling sites fitted well to a single correlation equation, which indicates that it was applicable to the estimation of E. coli concentrations regardless of the sampling sites. This method is simple, rapid, reliable, inexpensive, and high throughput, and is therefore useful for monitoring E. coli in RW.
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