Mechanistic insights into the effect of poly ferric sulfate on anaerobic digestion of waste activated sludge

Poly ferric sulfate (PFS), one of the typical inorganic flocculants widely used in wastewater management and waste activated sludge (WAS) dewatering, could be accumulated in WAS and inevitably entered in anaerobic digestion system at high levels. However, knowledge about its impact on methane production is virtually absent. This study therefore aims to fill this gap and provide insights into the mechanisms involved through both batch and long-term tests using either real WAS or synthetic wastewaters as the digestion substrates. Experimental results showed that the maximum methane potential and production rate of WAS was respectively retarded by 39.0% and 66.4%, whereas the lag phase was extended by 237.0% at PFS of 40 g per kg of total solids. Mechanism explorations exhibited that PFS induced the physical enmeshment and disrupted the enzyme activity involved in anaerobic digestion, resulting in an inhibitory state of the bioprocess of hydrolysis, acidogenesis, and methanogenesis. Furthermore, PFS's inhibition to hydrogenotrophic methanogenesis was much severer than that to acetotrophic methanogenesis, which could be supported by the elevated abundances of Methanosaeta sp and the dropped abundances of Methanobacterium sp in PFS-present digester, and probably due to the severe mass transfer resistance of hydrogen between the syntrophic bacteria and methanogens, as well as the higher hydrogen appetency of PFS-induced sulfate reducing bacteria. Among the derivatives of PFS, multinucleate and multichain-hydroxyl polymers and sulfate were unveiled to be the major contributors to the decreased methane potential, while the multinucleate and multichain-hydroxyl polymers were identified to be the chief buster to the slowed methane-producing rate and the extended lag time. (C) 2020 Elsevier Ltd. All rights reserved.

Automated summary

Poly ferric sulfate (PFS) has a significant impact on methane production in activated sludge, leading to decreased methane potential and production rate, as well as extended lag time. PFS causes physical enmeshment, disrupts enzyme activity, and inhibits the bioprocess of hydrolysis, acidogenesis, and methanogenesis.