Enzyme Catalyst Engineering toward the Integration of Biocatalysis and Chemocatalysis

Enzymatic catalysis, which has been driving biological processes in a green, mild, and efficient manner for billions of years, is increasingly being used in industrial processes to manufacture chemicals, pharmaceuticals, and materials for human society. Since enzymes were discovered, strategies to adapt enzymes for use as catalysts for industrial processes, such as chemical modification, immobilization, site-directed mutagenesis, directed evolution of enzymes, artificial metalloenzymes, and computational design, have been continuously pursued. In contrast to these strategies, editing enzymes to easily integrate biocatalysis with chemocatalysis is a potential way to apply enzymes in industry. Enzyme catalyst editing focuses on fine-tuning the microenvironment surrounding the enzyme or achieving a new catalytic function to construct better biocatalysis under nonnatural conditions for the enzyme.

Automated summary

Enzymatic catalysis, a green and efficient process that has been driving biological processes, is increasingly being used in industrial processes. Editing enzymes to integrate biocatalysis with chemocatalysis is a potential approach to improve catalytic efficiency and adaptability in nonnatural conditions.