4.6 Article

SARS-CoV-2-specific Cell-mediated Immunity in Kidney Transplant Recipients Recovered From COVID-19

期刊

TRANSPLANTATION
卷 105, 期 6, 页码 1372-1380

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LIPPINCOTT WILLIAMS & WILKINS
DOI: 10.1097/TP.0000000000003672

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  1. Instituto de Salud Carlos III (ISCIII), Spanish Ministry of Science and Innovation [COV20/00181]
  2. European Development Regional Fund A way to achieve Europe
  3. ISCIII, Spanish Ministry of Science and Innovation [CP18/00073, CM19/00120]

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Although still exploratory and limited by small sample size, the present study suggests that a substantial proportion of kidney transplant recipients exhibited detectable SARS-CoV-2-specific cell-mediated immunity after 6 months from COVID-19 diagnosis.
Supplemental Digital Content is available in the text. Background.The magnitude and kinetics of severe acute respiratory syndrome coronavirus 2-specific cell-mediated immunity (SARS-CoV-2-CMI) in kidney transplant (KT) recipients remain largely unknown.Methods.We enumerated SARS-CoV-2-specific interferon-gamma-producing CD69+ CD4+ and CD8+ T cells at months 4 and 6 from the diagnosis of coronavirus disease 2019 (COVID-19) in 21 KT recipients by intracellular cytokine staining. Overlapping peptides encompassing the SARS-CoV-2 spike (S) glycoprotein N-terminal 1- to 643-amino acid sequence and the membrane protein were used as stimulus. SARS-CoV-2 IgG antibodies targeting the S1 protein were assessed by ELISA at month 6.Results.Detectable (>= 0.1%) SARS-CoV-2-specific CD4+ T-cell response was found in 57.1% and 47.4% of patients at months 4 and 6. Corresponding rates for CD8+ T cells were 19.0% and 42.1%, respectively. Absolute SARS-CoV-2-specific T-cell counts increased from month 4 to month 6 in CD8+ (P = 0.086) but not CD4+ subsets (P = 0.349). Four of 10 patients with any detectable response at month 4 had lost SARS-CoV-2-CMI by month 6, whereas 5 of 9 patients mounted SARS-CoV-2-CMI within this period. All but 2 patients (89.5%) tested positive for SARS-CoV-2 IgG. Patients lacking detectable SARS-CoV-2-specific CD4+ response by month 6 were more likely to be under tacrolimus (100.0% versus 66.7%; P = 0.087) and to have received tocilizumab for the previous COVID-19 episode (40.0% versus 0.0%; P = 0.087).Conclusions.Although still exploratory and limited by small sample size, the present study suggests that a substantial proportion of KT recipients exhibited detectable SARS-CoV-2-CMI after 6 months from COVID-19 diagnosis.

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