期刊
TRANSBOUNDARY AND EMERGING DISEASES
卷 69, 期 2, 页码 598-608出版社
WILEY-HINDAWI
DOI: 10.1111/tbed.14026
关键词
ELISA; epidemiology; genome; porcine torovirus; S1 protein; serology
资金
- National Key Research and Development Program of China [2017YFD0500104]
In this study, a novel serological detection method based on PToV envelope spike subunit 1 (S1) protein was developed for the first time, leading to the first seroepidemiological survey of PToV in China. The study revealed a relatively high incidence of PToV seropositivity in pigs of different ages, providing the first serological evidence for PToV infection in pigs from China.
Toroviruses (ToVs), closely related but genetically distinct from coronaviruses, are known to infect horses, cows, pigs, goats and humans, mainly causing enteritic disorders. However, due to the lack of an adaptive culture system, porcine ToV (PToV) has received less attention. In this study, we developed a novel serological detection method based on the PToV envelope spike subunit 1 (S1) protein for the first time, and compared it to an existing indirect enzyme-linked immunosorbent assay (ELISA) based on the nucleocapsid protein. By using the S1-based ELISA, we carried out the first seroepidemiological survey of PToV in China, assaying both specific IgG and IgA responses in 1,037 serum samples collected from diarrheic pigs in eastern China. There was a relatively high incidence of seropositivity in pigs of different ages, especially one-week-old piglets and sows (78% and 43%), the former probably reflecting maternal antibodies. Furthermore, 3/20 (15%) of faecal samples collected from one PToV-seropositive swine herd in Zhejiang province tested positive by RT-PCR. The complete PToV genome was sequenced from one of these samples, and its phylogenetic relationship with other full-length PToV sequences available in GenBank was determined. Our data provide the first serological evidence for PToV infection in pigs from China, which will help elucidate the potential pathogenicity of PToV in pigs.
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