期刊
TRANSBOUNDARY AND EMERGING DISEASES
卷 69, 期 2, 页码 538-548出版社
WILEY-HINDAWI
DOI: 10.1111/tbed.14017
关键词
detection; genome organization; phylogenetic analysis; porcine circovirus 4
资金
- Korea Science and Engineering Foundation (KOSEF) - Korean government [2020R1I1A1A01054539]
- National Research Foundation of Korea [2020R1I1A1A01054539] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
The novel and unclassified porcine circovirus 4 (PCV4) was first reported in China in 2019 and has been found to be actively circulating in pigs in both China and Korea. The Korean PCV4 strain shares high genetic similarity with other known PCV4 strains. The study also identified key genomic features and predicted essential replication elements for circoviruses in PCV4 strains, contributing to a better understanding of the genetic relationships between PCV4 and other CRESS DNA viruses.
Porcine circovirus 4 (PCV4), a novel and unclassified member of the genus Circovirus, was first reported in China in 2019. Aiming to provide more evidence about the active circulation of PCV4, this study screened 335 pooled internal organs and detected the virus (i) at a rate of 3.28%, (ii) from both clinically healthy and clinically sick pigs of various age groups, and (iii) in six out of nine provinces of Korea. The complete genomic sequence of the Korean PCV4 strain (E115) was 1,770 nucleotides in length and had 98.5%-98.9% identity to three PCV4 strains currently available at GenBank. Utilizing a set of bioinformatic programs, it was revealed that the Korean PCV4 strain contained several genomic features of (i) a palindrome stem-loop structure with a conserved nonanucleotide, (ii) packed overlapping ORFs oriented in different directions and (iii) two intergenic regions in between genes encoding the putative replication-associated protein (Rep) and capsid (Cap) proteins. This study also predicted the presence of essential elements for the replication of circoviruses in all PCV4 strains, for example the origin of DNA replication, endonuclease and helicase domains of Rep, and the nuclear localization signal on the putative Cap protein. Finally, based on the phylogeny inferred from sequences of the putative Rep protein, this study further clarified the genetic relationships between PCV4 and other CRESS DNA viruses in general and circoviruses in particular.
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