期刊
TRANSACTIONS OF THE ROYAL SOCIETY OF TROPICAL MEDICINE AND HYGIENE
卷 115, 期 9, 页码 1080-1085出版社
OXFORD UNIV PRESS
DOI: 10.1093/trstmh/traa173
关键词
Acinetobacter baumannii; antimicrobial resistance mechanisms; bloodstream infections; New Delhi metallo-beta-lactamase 1; Tanzania
资金
- University of Bergen, Bergen, Norway
- AMR CrossCouncil Initiative through Medical Research Council, a Council of UK Research and Innovation [MR/S004793/1]
- Medical Research Council [MR/N013514/1]
- National Institute for Health Research [NIHR200632]
This study reported the first New Delhi metallo-beta-lactamase-producing isolates of Acinetobacter baumannii from Tanzania. The two isolates harbored blaNDM-1 gene, highlighting the importance of ISAba125 in the spread of blaNDM-1 in A. baumannii.
Background: We aimed to characterise the genetic determinants and context of two meropenem-resistant clinical isolates of Acinetobacter baumannii isolated from children hospitalised with bloodstream infections in Dar es Salaam, Tanzania. Methods: Antimicrobial susceptibility was determined by disc diffusion E-test and broth microdilution. Genomes were completed using a hybrid assembly of Illumina and Oxford Nanopore Technologies sequencing reads and characterisation of the genetic context of resistance genes, multi-locus sequence types (STs) and phylogenetic analysis was determined bioinformatically. Results: Twelve A. baumannii were isolated from 2226 blood cultures, two of which were meropenem-resistant. The two meropenem-resistant isolates, belonging to distinct STs, ST374 and ST239, were found to harbour blaNDM-1, which was chromosomally located in isolate DT0544 and plasmid-located in isolate DT01139. The genetic environment of blaNDM-1 shows the association of insertion sequence ISAba125 with blaNDM-1 in both isolates. Both isolates also harboured genes conferring resistance to other beta-lactams, aminoglycosides and cotrimoxazole. Conclusions: This is the first report of New Delhi metallo-beta-lactamase-producing isolates of A. baumannii from Tanzania. The genetic context of blaNDM-1 provides further evidence of the importance of ISAba125 in the spread of blaNDM-1 in A. baumannii. Local surveillance should be strengthened to keep clinicians updated on the incidence of these and other multidrug-resistant and difficult-to-treat bacteria.
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