Proteins from Rhinella jimi parotoid gland secretion: A comprehensive analytical approach

Toad skin secretions are sources of complex mixtures of bioactive compounds, such as proteins and peptides. Rhinella jimi species is a common toad in the Brazilian northeast, considered by only a few known studies. The experimental design was applied to optimize the protein extraction method from R. jimi parotoid gland secretions. The optimum condition was using 100 mmol L-1 Tris-HCl buffer pH 7.2 under vortexing for 5 min. The FTIR analysis combined with PCA revealed high-protein purity of the extracts, confirming the success of the proposed extraction method. The total protein concentration by the Bradford method was 102.4 and 66.5 mg g(-1) on toad poisons from Teresina and Picos, respectively. The comparative proteomic analysis using HPLC-SEC-DAD and 1D SDS-PAGE revealed significant differences in protein abundance. HMW biomolecules showed greater abundance in toads from Teresina, while LMW protein species were more abundant in toads from Picos. The significant difference in amphibian proteome can be attributed to the edaphoclimatic conditions of their habitat. The cytotoxicity of the protein extract from Teresina was higher on the tumor cell lines 4T1 and CT26.WT. These new findings are fundamental for future studies the on identity and biological activity of biomolecules from this noble sample.

Automated summary

Toad skin secretions are rich sources of bioactive compounds, with Rhinella jimi species in the Brazilian northeast being a common toad with limited known studies. An experimental design optimized the protein extraction method from R. jimi parotoid gland secretions, revealing differences in protein abundance between toads from Teresina and Picos. The protein extract from Teresina showed higher cytotoxicity on tumor cell lines 4T1 and CT26.WT, indicating potential for future research on the biological activity of biomolecules from this species.