期刊
TALANTA
卷 224, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.talanta.2020.121869
关键词
Graphene quantum dots; Poly(N-isopropylacrylamide); Mixed-mode stationary phase; Reversed-phase/hydrophilic interaction; Chromatographic separation
资金
- National Natural Science Foundation of China [21804112]
- Project of Department of Education of Sichuan Province [18ZA0527]
- talent introduction program of Southwest Medical University [090300040040]
- Southwest Medical University [2017-ZRQN0118]
- Sichuan Science and Technology Program [2020YFS0313]
The study polymerized poly(N-isopropylacrylamide) onto the surface of graphene quantum dots (GQDs) functionalized silica as packing materials, demonstrating a column with reversed-phase and hydrophilic interaction modes for separating target analytes with different Log P values. The Sil-GQDs-PNIPAAm stationary phase shows potential for improving the analysis range and performance of traditional phases.
The poly(N-isopropylacrylamide) (NIPAAm) was first polymerized onto the surface of graphene quantum dots (GQDs) functionalized silica as packing materials via reversible addition-fragmentation chain transfer (RAFT) polymerization reaction, which can expand the interaction modes between stationary phase and analytes. A series of characteristic methods were selected to estimate the chemical bonding results of silica, involving Fourier transform infrared spectroscopy (FT-IR), elemental analysis (EA), thermogravimetric analysis (TGA) and scanning electron microscope (SEM). The prepared column can exhibit reversed-phase and hydrophilic interaction modes, which were demonstrated by the retention of eight kinds of target analytes with different Log P values. The column was then applied to separate banlangen granules and further verified by HPLC tandem time-of-flight mass spectrometry (HPLC/QTOF-MS). In conclusion, Sil-GQDs-PNIPAAm stationary phase improved the analysis range and performance of traditional phases, exhibiting flexible selectivity and application prospect for both hydrophobic and hydrophilic analytes.
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