期刊
STRUCTURE
卷 29, 期 8, 页码 886-+出版社
CELL PRESS
DOI: 10.1016/j.str.2021.01.010
关键词
-
资金
- NIH [R35 GM122518, R01 GM110639, R01 GM120574]
The ET domain of BRD3 interacts with viral and host proteins through similar beta-sheet formations. N-15 relaxation studies reveal a restricted flexibility in the linker region, affecting its orientation in the protein complexes. The complex of the ET-binding peptide of host NSD3 protein and the BRD3 ET domain shows a different orientation compared to viral protein complexes.
The extraterminal (ET) domain of BRD3 is conserved among BET proteins (BRD2, BRD3, BRD4), interacting with multiple host and viral protein-protein networks. Solution NMR structures of complexes formed between the BRD3 ET domain and either the 79-residue murine leukemia virus integrase (IN) C-terminal domain (IN329-408) or its 22-residue IN tail peptide (IN386-407) alone reveal similar intermolecular three-stranded beta-sheet formations. N-15 relaxation studies reveal a 10-residue linker region (IN379-388) tethering the SH3 domain (IN329-378) to the ET-binding motif (IN389-405):ET complex. This linker has restricted flexibility, affecting its potential range of orientations in the IN:nucleosome complex. The complex of the ET-binding peptide of the host NSD3 protein (NSD3148-184) and the BRD3 ET domain includes a similar three-stranded beta-sheet interaction, but the orientation of the beta hairpin is flipped compared with the two IN:ET complexes. These studies expand our understanding of molecular recognition polymorphism in complexes of ET-binding motifs with viral and host proteins.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据