Generation of induced pluripotent stem cell line (IPTi002-A) from an 87-year old sporadic Alzheimer's disease patient with APOE3 (ε3/ε3) genotype

Here, we described the generation of human induced pluripotent stem cells (iPSC) from peripheral blood mononuclear cells (PBMCs) of a 87-year-old female patient with sporadic Alzheimer's disease (sAD) having APOE3 (epsilon 3/epsilon 3) genotype. iPSC line were generated from PBMCs with four factors of OCT4, SOX2, c-MYC and KLF4 using episomal system. The pluripotency of the iPSC line was assessed by embryoid body (EB) formation. Flow cytometry analyses revealed >97% cells positive for the pluripotency markers NANOG, OCT4 and SSEA4. Furthermore, the iPSC line displayed a normal karyotype (46, XX). The iPSC line may provide valuable tools for the study of sAD pathogenesis.

Automated summary

In this study, human induced pluripotent stem cells (iPSCs) were successfully generated from peripheral blood mononuclear cells (PBMCs) of an 87-year-old female patient with sporadic Alzheimer's disease (sAD) and APOE3 genotype. The pluripotency of the iPSC line was confirmed through embryoid body formation and flow cytometry analyses. The iPSC line exhibited a normal karyotype and could serve as a valuable tool for studying sAD pathogenesis.