期刊
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
卷 249, 期 -, 页码 -出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.saa.2020.119211
关键词
Two-dimensional correlation spectroscopy (2DCOS); Residual convolutional neural network (Resnet); Deep learning; Bolete; Species discrimination; Application (APP)
类别
资金
- National Natural Science Foundation of China [32060570, 31660591]
- Yunnan Province Agricultural Basic Research Joint Special Project [2018FG001-101]
A new method using two-dimensional correlation spectroscopy (2DCOS) and deep learning for the species discrimination of bolete mushrooms was proposed in this study. The method achieved high accuracy in identifying different species and a smartphone application was developed for practical use.
Bolete is well-known and widely consumed mushroom in the world. However, its medicinal properties and nutritional are completely different from one species to another. Therefore, the consumers need a fast and effective detection method to discriminate their species. A new method using directly digital images of two-dimensional correlation spectroscopy (2DCOS) for the species discrimination with deep learning is proposed in this paper. In our study, a total of 2054 fruiting bodies of 21 wild-grown bolete species were collected in 52 regions from 2011 to 2014. Firstly, we intercepted 1750-400 cm(-1) fingerprint regions of each species from their mid-infrared (MIR) spectra, and converted them to 2DCOS spectra with matlab2017b. At the same time, we developed a specific method for the calculation of the 2DCOS spectra. Secondly, we established a deep residual convolutional neural network (Resnet) with 1848 (90%) 2DCOS spectral images. Therein, the discrimination of the bolete species using directly 2DCOS spectral images instead of data matric from the spectra was first to be reported. The results displayed that the respective identification accuracy of these samples was 100% in the training set and 99.76% in the test set. Then, 203 samples were accurately discriminated in 206 (10%) samples of external validation set. Thirdly, we employed t-SNE method to visualize and evaluate the spectral dataset. The result indicated that most samples can be clustered according to different species. Finally, a smartphone applications (APP) was developed based on the established 2DCOS spectral images strategy, which can make the discrimination of bolete mushrooms more easily in practice. In conclusion, deep learning method by using directly 2DCOS spectral image was considered to be an innovative and feasible way for the species discrimination of bolete mushrooms. Moreover, this method may be generalized to other edible mushrooms, food, herb and agricultural products in the further research. (C) 2020 Elsevier B.V. All rights reserved.
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