Novel spectrofluorimetric determination of brigatinib in bulk powder and human urine samples via ion-pair complex formation using eosin Y

The developed spectrofluorimetric method was successfully applied to the analysis of brigatinib (BRG) in its bulk powder form, and human urine sample. It is based on the investigation of the fluorescence spectrum behavior of the BRG-eosin Y complex. The relative fluorescence intensity (RFI) was recorded at 560 nm after excitation at 480 nm. The principle of the proposed method was thoroughly explained. All experimental parameters affecting method development were optimized. Moreover, the obtained results were fully discussed and statistically analyzed. The molar ratio method was applied to study the stoichiometric relationship between BRG and eosin Y complex. The method revealed a ratio of 1:3 for BRG-eosin Y afforded the highest RFI. The developed method was validated over the concentration range of 62.5-4000 ng mL(-1). The results were compared positively with the reported method. (C) 2020 Elsevier B.V. All rights reserved.

Automated summary

The developed spectrofluorimetric method based on BRG-eosin Y complex analysis was successfully applied to bulk powder form and human urine sample. The method was thoroughly explained, experiment parameters optimized, and results discussed and validated. The stoichiometric relationship between BRG and eosin Y complex was studied, with a 1:3 ratio resulting in the highest RFI.