An OliGreen-responsive fluorescence sensor for sensitive detection of organophosphorus pesticide based on its specific selectivity towards T-Hg2+-T DNA structure

In this paper, it was found that OliGreen emitted much stronger fluorescence in rigid T-Hg2+-T DNA structure than that in the presence of poly T. Thus, an OliGreen-responsive label-free fluorescent sensor was proposed for sensitive detection of organophosphorus pesticides (OPs) by constructing T-Hg2+-T DNA structure. OliGreen emits strong fluorescence in T-Hg2+-T structures. The rigid DNA structure of T-Hg2+-T is prone to be destroyed by thiocholine (TCh) that hydrolyzed by acetylcholinesterase (AChE) because of the high affinity of TCh with Hg2+. As a result, T-Hg2+-T DNA structure broke down and the fluorescence intensity of OliGreen decreased greatly. With the inhibition of AChE by OPs, fluorescence intensity of OliGreen remained strong because of the rigid T-Hg2+-T DNA structure. Thus, a turn-on fluorescent sensor which avoids synthesis of nanomaterials and complex label procedures is proposed based on the fluorescence intensity of OliGreen. DDVP were detected with a wide linear range from 0.005 to 25.0 ng/mL and the detection limit was 2.9 pg/mL, which is more sensitive than previously reported methods. (C) 2020 Elsevier B.V. All rights reserved.

Automated summary

It was found in this paper that OliGreen emits strong fluorescence in rigid T-Hg2+-T DNA structure compared to poly T. A label-free fluorescent sensor based on OliGreen was proposed for detecting organophosphorus pesticides by constructing T-Hg2+-T DNA structure. The sensor works by monitoring the fluorescence intensity change of OliGreen, which remains strong in the presence of OPs due to the rigid T-Hg2+-T DNA structure.