期刊
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
卷 247, 期 -, 页码 -出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.saa.2020.119145
关键词
Copper nanoclusters; Glutathione; Fluorescence quenching; Furazolidone
类别
资金
- Shanxi Applied Basic Research Project [201801D121257]
- Shanxi Province Science Foundation for Youths [201801D221142]
- College Students' Innovation Program of Taiyuan Normal University [CXCY2004]
- Science and Technology Innovation Project of Shanxi Province [2020L0499]
- Innovation and Entrepreneurship Training Project for College Students in Shanxi Province [2020486]
- Fund for Shanxi 1331 Project Collaborative Innovation Center [I018038]
A green method using GSH and ascorbic acid was developed to prepare Cu nanoclusters, which showed good water solubility, stability, and dispersibility. The method can be used for furazolidone detection in bovine serum and provides a rapid, easy, and ultra-sensitive fluorescence platform.
Herein, a facile, straightforward and green method was developed to prepare copper nanoclusters by using glutathione (GSH) as the protecting agent and ascorbic acid as the reducing agent. The glutathione-templated copper nanoclusters (GSH-Cu NCs) were characterized through fluorescence spectroscopy, UV-vis absorption spectroscopy, Fourier transform infrared (FT-IR) spectroscopy, transmission electron microscopy (TEM), X-ray photoelectron spectroscopy (XPS) and fluorescence lifetime analysis. The as-synthesized Cu NCs showed blue fluorescence with a peak centered at 426 nm. The Cu NCs had excellent water solubility, stability and dispersibility. Based on the inner filter effect and static quenching mechanism, Cu NCs were employed to detect furazolidone in bovine serum samples. Under optimal detection conditions, a good linear relationship was observed between F-0/F and the furazolidone concentration from 0.05 to 60 mu M. The detection limit (LOD) was 0.012 mu M. Furthermore, the fluorescence probe was successfully used in the quantification of furazolidone in bovine serum samples. In addition, this analytical method provides a rapid, easy and ultrasensitive fluorescence platform for the detection of furazolidone. (C) 2020 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据