Structure and dynamics of poly(methacrylic acid) and its interpolymer complex probed by covalently bound rhodamine-123

The dynamics and structural characteristics of polymethacrylic acid bound rhodamine-123 (PMAA-R123) and its interpolymer complex formed through hydrogen bonding between the monomeric units with poly(vinylpyrrolidone) were investigated using single molecular fluorescence studies. The time resolved fluorescence anisotropy decay of PMAA-R123 under acidic pH exhibits an associated anisotropy decay behavior characteristic of two different environments experienced by the fluorophore with one shorter and another longer rotational correlation time. The anisotropy decay retains normal bi-exponential behavior under neutral pH. Fluorescence correlation spectroscopic investigation reveals that the attached fluorophore undergoes hydrolysis under basic condition which results in the release of the fluorophore from the polymer backbone. Shrinkage in the hydrodynamic radius of PMAA is observed on addition of the complementary polymer PVP which is attributed to the formation compact solubilized nanoparticle like aggregates. The size of particle further decreases on the addition of NaCl. The detailed results show that these complexes have potential for use as drug-delivery system under physiological conditions. (C) 2020 Elsevier B.V. All rights reserved.

Automated summary

The dynamics and structural characteristics of the PMAA-R123 and its interpolymer complex with PVP were investigated using single molecular fluorescence studies. Different environments experienced by the fluorophore under acidic pH resulted in distinct fluorescence anisotropy decay behaviors, while hydrolysis of the fluorophore under basic conditions led to its release from the polymer backbone. These complexes have potential for drug-delivery systems under physiological conditions.