Biomass production and secondary metabolite identification in callus cultures of Coryphantha macromeris (Engelm.) Britton & Rose (Cactaceae), a traditional medicinal plant

Plant cell, tissue, and organ culture have become a powerful technology for the production of biomass, and for the research and biosynthesis of a variety of secondary metabolites. In this work, we report an efficient method for friable callus induction applied to the medicinal cactus Coryphantha macromeris, its kinetic behavior, and its phytochemical profile, assessed at the maximum biomass production phase. Callus cultures were obtained from stem discs inoculated on Murashige and Skoog medium (MS) supplemented with 6-benzylaminopurine (BAP; 2.2 mu M) and picloram (4.14 mu M). The highest biomass production (20.65 g DW L-1) was achieved at nine weeks of culture. Then, the phytochemical profile was analyzed using Ultra-High-Performance Liquid Chromatography-tandem Mass Spectrometry (UHPLC-PDA-HESI-Orbitrap-MS/MS). Chromatographic and mass spectrometric analyses indicated the presence of 61 metabolites, with 52 being identified. Among these compounds, 11 organic acids, 16 phenolic acids, 8 flavonoids, and 17 metabolites of different classes were identified. Our results could significantly contribute to the current knowledge of tissue culture of cacti species, as well as the potential applications of the in vitro callus culture of C. macromeris. Furthermore, we report the presence of some metabolites in cell culture of cacti species and their fragmentation pattern for the first time. (C) 2020 SAAB. Published by Elsevier B.V. All rights reserved.

Automated summary

Plant cell, tissue, and organ culture have been utilized as a powerful technology for biomass production and secondary metabolite biosynthesis. This study presents an efficient method for inducing friable callus in the medicinal cactus Coryphantha macromeris, analyzing its kinetic behavior and phytochemical profile. The research achieved high biomass production and identified multiple metabolites, contributing to the understanding of tissue culture and potential applications of the cactus species.