期刊
SENSORS AND ACTUATORS B-CHEMICAL
卷 329, 期 -, 页码 -出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2020.129118
关键词
H+-selective solid electrode; RT-LAMP; HIV-1; Point-of-care; Screen-printed graphene electrode
资金
- National Key R&D Program of China [2018YFC0115705]
- National Natural Science Foundation of China [51675517, 61874133, 61901469, 22005331]
- Key Research and Development Program of Jiangsu Province [BE2020768, BE2019684, BE2018080]
- Jihua Laboratory Foundation [X190181TD190]
- Youth Innovation Promotion Association CAS [2019322, 2018360, Y201856]
- STS Projects of the Chinese Academy of Sciences [KFJ-STS-SCYD-217, KFJ-STSZDTP-061]
- Instrument Developing Project of the Chinese Academy of Sciences [YJKYYQ20190057]
- Science and Technology Development Program of Suzhou [SYG201907]
- Jiangsu Provincial Policy Guidance Program [BZ2019069]
- Funding Projects of 20 items for University and College in Jinan [2018GXRC030]
The newly developed H+ -RT-LAMP sensor is a novel hydrogen ion-selective sensor for highly sensitive detection of HIV-1, widely applied for on-site detection.
Development of a simple, sensitive and specific method for HIV-1 assay is demanded. In some emergency situations, a portable reverse transcription loop-mediated isothermal amplification (RT-LAMP) platform is necessary. A novel hydrogen ion-selective RT-LAMP (H+ -RT-LAMP) sensor was developed for highly sensitive HIV-1 using hydrogen ion concentration as a signal producing compound. The H+ -RT-LAMP sensor was based on screen-printed with carbon-doped graphene conductive layer, carbon nanotubes (CNTs) solid electrolyte layer, and reference electrode KCl functional layer, which provided superior electrical signals, high sensitivity and stability for HIV-1 detection. The rate of hydrogen ion production was related to the viral load (VL) during the amplification of HIV-1. The sensitive HIV-1 RNA detection range was achieved from 0 copies/mu L to 10(5) copies/mu L. The proposed H+ -RT-LAMP sensor was applied to detect HIV-1 RNA in real human serum. 57 samples were detected and satisfactory results were obtained. Compared with commercial instruments, the detection results of H+ -RT-LAMP sensor demonstrated a perfect concordance rate of 96.5 %, and the limit of detection (LOD) for the clinical samples was 10 copies per tube. Time to get the result is from 10 min to 25 min. Therefore, the proposed H+ -RT-LAMP sensor was particularly suitable as a point-of-care device for the on-site detection of HIV-1.
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