期刊
SENSORS AND ACTUATORS B-CHEMICAL
卷 329, 期 -, 页码 -出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2020.129243
关键词
Electrochemical impedance spectroscopy; Biosensor; Insect odorant receptor; Lipid nanodiscs; Odorant sensor; Neutron reflectometry
资金
- Better Border Biosecurity (B3) grant [P/321022/06]
- New Zealand Institute for Plant and Food Research Limited (PFR) SSIF grant [P/321061/02]
- Australian Institute of Nuclear Science and Engineering (AINSE) Postgraduate research award (PGRA)
- Australia's Nuclear Science and Technology Organisation (ANSTO) [7364]
This study presents an electrochemical sensing methodology using an insect odorant receptor (iOR) as the biological recognition element for detection of odorant compounds. The fruit fly Drosophila melanogaster's Or22a was used as a model receptor and shown to be functionally active towards its known ligand (ethyl hexanoate). The EIS sensor exhibited high sensitivity and specificity towards ethyl hexanoate with a detection limit of 5.5 fM, with neutron reflectometry studies providing evidence of conformational changes in the receptor upon ligand binding.
An electrochemical sensing methodology using an insect odorant receptor (iOR) as the biological recognition element for detection of odorant compounds is presented. Or22a from the fruit fly Drosophila melanogaster (DmOr22a) was used as a model receptor for the study. DmOr22a in phospholipid nanodisc preparations was shown to be functionally active toward its known ligand (ethyl hexanoate), as measured by electrochemical impedance spectroscopy (EIS). iOR nanodiscs covalently immobilized on a gold substrate were further characterized by atomic force microscopy (AFM), quartz crystal microbalance with dissipation monitoring (QCM-D) and neutron reflectometry in order to study surface modification and understand the underlying mechanisms associated with ligand binding. The EIS sensor exhibited high sensitivity and specificity towards ethyl hexanoate with a detection limit of 5.5 fM. Neutron reflectometry studies provided evidence to support conformational changes in the receptor upon ligand binding. The EIS based detection strategy provides a facile, sensitive and fast method for detecting odorant compounds and paves the way for further development of odorant biosensors.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据