期刊
SENSORS AND ACTUATORS B-CHEMICAL
卷 328, 期 -, 页码 -出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2020.129068
关键词
Radiofrequency and microwave chips; Self-assembly; Proteins; Tubulin; Microtubules; Dielectric spectroscopy; High-frequency biosensors
资金
- Czech Science Foundation [20-06873X]
- COST Actions [CA1521]
- Czech Academy of Science [SAV-18-11]
- Slovak Academy of Science [SAV-18-11]
A novel method for monitoring tubulin self-assembly states using microwave lab-on-a-chip technology was proposed and validated experimentally, demonstrating the effectiveness of this approach in detecting the assembly of tubulin proteins into microtubules.
Self-assembly is at the heart of many promising nanoscience technologies as well as at the core of life processes. Tubulin proteins self-assemble into microtubules, tube-like structures that are essential in cellular functions such as cell division and intracellular transport and also a major target in cancer therapies. Therefore, it is crucial to develop efficient methods for monitoring tubulin self-assembly at the molecular level. To mitigate the limitations of current methods, we propose a new approach based on a microwave lab-on-a-chip method to monitor tubulin self-assembly states. To that end, we designed a dedicated microwave platform with integrated microfluidics with a sensing volume of < 30 nL. In parallel, we used a standard bulk light-scattering-based method to assess the tubulin self-assembly and to validate the microwave chip results. Using our chip, for the first time, we demonstrate that the self-assembly state of tubulin into microtubules can be monitored using microwave microfluidics technology. Our results introduce a novel label-free electromagnetic monitoring and analytical method for bionanotechnology and biomedicine applications that can potentially be integrated into advanced microscopy systems.
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