Elucidating the Quenching Mechanism in Carbon Dot-Metal Interactions-Designing Sensitive and Selective Optical Probes

Overexposure to metals has significant adverse effects on human and animal health coupled with nefarious consequences to the environment. Sensitive tools to measure low contaminant levels exist, but often come at a high cost and require tedious procedures. Thus, there exists a need for the development of affordable metal sensors that can offer high sensitivity and selectivity while being accessible on a global scale. Here, carbon dots, prepared in a one-pot synthesis using glutathione and formamide, have been developed as dual fluorescent metal sensing probes. Following extensive characterization of their physico-chemical properties, it is demonstrated that dual fluorescence can be exploited to build a robust ratiometric sensor with low-ppb detection sensitivity in water. This investigation shows that these optical probes are selective for Pb2+ and Hg2+ ions. Using steady-state and dynamic optical characterization techniques, coupled with hard and soft acid-base theory, the underlying reason for this selective behavior was identified. These findings shed light on the nature of metal-carbon dot interactions, which can be used to tailor their properties to target specific metal ions. Finally, these findings can be applicable to other fluorescent nanoparticle systems that are targeted for development as metal sensors.

Automated summary

Carbon dots prepared using glutathione and formamide as dual fluorescent metal sensing probes show selective behavior towards Pb2+ and Hg2+ ions, leading to the development of a robust ratiometric sensor with low-ppb detection sensitivity in water. Characterization techniques and acid-base theory reveal insights into the nature of metal-carbon dot interactions, which can be used to tailor properties for targeting specific metal ions and applicable to other fluorescent nanoparticle systems for metal sensing applications.