Rapid Analysis for Staphylococcus aureus via Microchip Capillary Electrophoresis

Staphylococcus aureus (S. aureus) is one of the most common pathogens for nosocomial and community infections, which is closely related to the occurrence of pyogenic and toxic diseases in human beings. In the current study, a lab-built microchip capillary electrophoresis (microchip CE) system was employed for the rapid determination of S. aureus, while a simple-to-use space domain internal standard (SDIS) method was carried out for the reliable quantitative analysis. The precision, accuracy, and reliability of SDIS were investigated in detail. Noted that these properties could be elevated in SDIS compared with traditional IS method. Remarkably, the PCR products of S. aureus nuc gene could be identified and quantitated within 80 s. The theoretical detection limit could achieve a value of 0.066 ng/mu L, determined by the using SDIS method. The current work may provide a promising detection strategy for the high-speed and highly efficient analysis of pathogens in the fields of food safety and clinical diagnosis.

Automated summary

The lab-built microchip capillary electrophoresis system and space domain internal standard method were used for rapid and reliable detection and quantification of S. aureus. The SDIS method showed higher precision and accuracy compared to traditional methods, allowing for quick identification and quantitation of the pathogen.