Alteration of neuro-dopamine and steroid hormone homeostasis in wild Bank voles in relation to tissue concentrations of PFAS at a Nordic skiing area

Perlluoroalkyl substances (PEAS) are contaminants that are applied in a wide range of consumer products, including ski products. The present study investigated the neuro-dopamine (DA) and cellular steroid hormone homeostasis of wild Bank voles (Myodes glareolus) from a skiing area in Norway (Trondheim), in relation to tissue concentrations of PFAS. We found a positive association between brain DA concentrations and the concentration of several PFAS, while there was a negative association between PFAS and dopamine receptor 1 (dr1) mRNA. The ratio between DA and its metabolites (3,4-dihydroxyphenylacetic acid: DOPAC and homovanillic acid: HVA) showed a negative association between DOPAC/DA and several PFAS, suggesting that PFAS altered the metabolism of DA via monoamine oxidase (Mao). This assumption is supported by an observed negative association between mao mRNA and PFAS. Previous studies have shown that DA homeostasis can indirectly regulate cellular estrogen (E2) and testosterone (T) biosynthesis. We found no association between DA and steroid hormone levels, while there was a negative association between some PFAS and T concentrations, suggesting that PFAS might affect T through other mechanisms. The results from the current study indicate that PEAS may alter neuro-DA and steroid hormone homeostasis in Bank voles, with potential consequences on reproduction and general health. (C) 2020 The Author(s). Published by Elsevier B.V.

Automated summary

PEAS may alter neuro-dopamine and steroid hormone homeostasis in Bank voles from a skiing area in Norway, potentially affecting reproduction and general health. The study found a positive association between brain dopamine concentrations and several PFAS, as well as a negative association between PFAS and dopamine receptor 1 mRNA. Additionally, PFAS were found to affect dopamine metabolism and testosterone concentrations in the voles.