On the effects of iron ore tailings micro/nanoparticles in embryonic and larval zebrafish (Danio rerio)

Iron ore tailings (IOT) represent a major problem in the mining industry worldwide due to large volumes of waste disposed in mine sites. IOT are exposed to the environment and subjected to wind and water dispersion, even under non-catastrophic scenarios as dam collapses, and the effects of these particles to the biota are still mostly unknown. This work aimed to prepare and to characterize a suspension containing the finest (micro/ nano range) particles of IOT and to evaluate its effects on development and behavior of zebrafish (Danio rerio), at both embryonic and larval stages. IOT suspension comprised 37 mg L-1 of a multi-mineral material mainly composed by hematite and quartz, in a size-range of 33-1400 nm. Regarding in vivo toxicological assays, no robust alterations were recorded in functional, morphological and behavioral end-points analyzed, although a significant adhesion of IOT particles on zebrafish chorion was observed, without a prejudice of embryo hatching. Under applied conditions, iron ore particles did not present harmful effects to the initial stages of zebrafish development, and the particle size range and potential interactions with SiO2 content might be behind such effect. (C) 2020 Elsevier B.V. All rights reserved.

Automated summary

The study found that iron ore tailings particles did not have significant harmful effects on the development and behavior of zebrafish embryos and larvae, although particle adhesion on the chorion was observed. Therefore, the particle size range and potential interactions with SiO2 content may contribute to explaining this effect.