The rs6983267 SNP and long non-coding RNA CARLo-5 are associated with endometrial carcinoma

The single nucleotide polymorphism (SNP) rs6983267 and cancer-associated region long non-coding RNA (CARLo-5) are associated with various human cancers. This study aimed to investigate the expression of CARLo-5 in endometrial carcinoma (EC) and its relationship with clinicopathological features and patient survival. The association of the rs6983267 SNP with EC risk and its involvement in the regulation of CARLo-5 expression in EC were investigated. The rs6983267 SNP was genotyped by polymerase chain reaction (PCR) and ligase detection reaction in 543 EC patients and 584 controls. The expression of CARLo-5 in 108 EC tissues and 66 normal endometrial tissues (NETs) was determined using quantitative real-time PCR. The genotype and allele distributions of the rs6983267 SNP differed significantly between patients and controls. There was a significant correlation between the rs6983267 genotypes and lymph node metastasis of EC patients (P=0.026). CARLo-5 expression was significantly higher in EC tissues than in NETs (P<0.001) and significantly associated with FIGO stage (P=0.029) and lymph node metastasis (P=0.030). Patients with high CARLo-5 expression had significantly shorter overall survival than those with low CARLo-5 expression (P=0.003). The rs6983267 genotype was significantly correlated with CARLo-5 expression (P<0.05). In conclusion, CARLo-5 was identified as a pro-oncogenic lncRNA that may play an important role in EC progression and represent a prognostic marker for EC. The expression of CARLo-5 was significantly correlated with the rs6983267 genotype associated with increased susceptibility to EC. Environ. Mol. Mutagen. 57:508-515, 2016. (c) 2016 Wiley Periodicals, Inc.