期刊
REPRODUCTION IN DOMESTIC ANIMALS
卷 56, 期 5, 页码 744-753出版社
WILEY
DOI: 10.1111/rda.13913
关键词
apoptosis; corpus luteum; luteal steroidogenic cells; luteinizing hormone
资金
- Directorate of Research and Community Service, University of Indonesia
- International Research Collaboration Grant [NKB-1944/UN2.R3.1/HKP.05.00/2019]
Our study indicates that LH prolongs the lifespan of the corpus luteum through an anti-apoptotic mechanism by increasing cell viability and suppressing the expression of apoptosis-related genes and proteins.
The fate of the corpus luteum, a transient endocrine gland formed and degraded during an oestrous cycle, is decided by various physiological factors, such as luteinizing hormone (LH). As a stimulator of progesterone, LH is known to maintain corpus luteum functional and structural integrity by inhibiting apoptosis, a programmed cell death. Therefore, we aim to investigate its action during the mid-luteal phase hypothesized that LH suppresses the death mechanism of bovine luteal steroidogenic cells (LSC) by analysing the expression of proteins involved. Cultured bovine LSC obtained from corpus luteum were treated for 24 hr with recombinant TNF and IFNG in the presence or absence of LH. The result showed that LH proved to have a protective effect by increased cell viability (p < .05) and prevented DNA fragmentation (p < .05), as demonstrated by the WST-1 colorimetric assay and TUNEL assay. Expression analysis of mRNA and protein levels showed that LH altered the expression of BCL2 (p < .05), CASP3 (p < .05), FAS (p < .05), and BAX (p < .05) to support cell survival. In conclusion, our study suggests that LH prolongs the corpus luteum life span through the anti-apoptotic mechanism by increasing cell viability and suppressing apoptosis-related genes and protein expression.
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