期刊
REPRODUCTION
卷 161, 期 2, 页码 111-122出版社
BIOSCIENTIFICA LTD
DOI: 10.1530/REP-20-0415
关键词
-
资金
- National Key R&D Program of China [2018YFD0501001]
- Natural Science Foundation of Shaanxi Province, China [2019JQ-430]
The study found that human sperm tRNA(Gln-TTG) derived small RNAs are closely associated with sperm quality and participate in human embryo development. Gln-TTGs may influence embryonic genome activation by modulating noncoding RNA processing.
Infertility caused by male factors is routinely diagnosed by assessing traditional semen parameters. Growing evidence has indicated that the tsRNAs carried in sperm act as epigenetic factors and potential biomarkers for the assessment of sperm quality. We recently demonstrated that tRNA(Gln-TTG) derived small RNAs played notable roles in the first cleavage of a porcine embryo. However, the function of human sperm tRNA(Gln-TTG) derived small RNAs as a diagnostic biomarker and its role in early embryo development remains unclear. In this study, we found that human sperm tRNA(Gln-TTG) derived small RNAs were highly associated with sperm quality. By microinjecting the antisense sequence into human tripronuclear (3PN) zygotes followed by single-cell RNA-sequencing, we found that human sperm tRNA(Gln-TTG) derived small RNAs participated in the development of a human embryo. Furthermore, Gln-TTGs might influence embryonic genome activation by modulating noncoding RNA processing. These findings demonstrated that human sperm tRNA(Gln-TTG )derived small RNAs could be potential diagnostic biomarkers and could be used as a clinical target for male infertility.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据