期刊
出版社
NATL ACAD SCIENCES
DOI: 10.1073/pnas.2008731118
关键词
antimicrobial resistance; mobile genetic elements; horizontal gene transfer; whole genome; long-reads sequencing
资金
- University of Hong Kong
- State Key Laboratory of Marine Pollution Seed Collaborative Research Fund [SCRF/0023]
- Research Grants Council of Hong Kong Special Administrative Region, China [T21-705/20-N]
The study found that plasmid-borne antimicrobial resistance genes are mainly enriched in conjugative plasmids. Scientists reported the discovery of a massive insertion sequence-associated antimicrobial resistance gene transfer network, suggesting a general evolutionary mechanism for horizontal gene transfer mediated by the interaction between conjugative plasmids and ISs. Experimental results confirmed the importance of these interactions in aiding the horizontal transfer and expanding the genetic range of antimicrobial resistance genes within complex microbial communities.
It is well established that plasmids play an important role in the dissemination of antimicrobial resistance (AMR) genes; however, little is known about the role of the underlying interactions between different plasmid categories and other mobile genetic elements (MGEs) in shaping the promiscuous spread of AMR genes. Here, we developed a tool designed for plasmid classification, AMR gene annotation, and plasmid visualization and found that most plasmid-borne AMR genes, including those localized on class 1 integrons, are enriched in conjugative plasmids. Notably, we report the discovery and characterization of a massive insertion sequence (IS)-associated AMR gene transfer network (245 combinations covering 59 AMR gene subtypes and 53 ISs) linking conjugative plasmids and phylogenetically distant pathogens, suggesting a general evolutionary mechanism for the horizontal transfer of AMR genes mediated by the interaction between conjugative plasmids and ISs. Moreover, our experimental results confirmed the importance of the observed interactions in aiding the horizontal transfer and expanding the genetic range of AMR genes within complex microbial communities.
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