期刊
POULTRY SCIENCE
卷 100, 期 5, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.psj.2021.101046
关键词
acid substitution; antimicrobial activity; cathelicidin; host defense peptide; SNP
资金
- Advanced Research Project Type A, Tokyo University of Agriculture [02]
- MEXT-Supported Program for the Strategic Research Foundation at Private Universities [S1311017]
The study aimed to clarify the polymorphisms in CATHs in Japanese quail, providing guidance for improving innate immune response in poultry; amino acid substitutions accompanied by changes in antibacterial activity were found in avian CATHs.
Japanese quail is a low-fat, meat-bird species exhibiting high disease resistance. Cathelicidins (CATHs) are host defense peptides conserved across numerous vertebrate species that play an important role in innate immunity. The activity of host defense peptides can be affected by amino acid substitutions. However, no polymorphisms in avian CATH genes have been reported to date. The aim of this study was to clarify the poly-morphisms in CATHs in Japanese quail. DNA for genomic analyses was extracted from the peripheral blood of 99 randomly selected quail from 6 inbred lines. A total of 6, 4, 6, and 4 CjCATH1,-2,-3, and-B1 alleles were identified, respectively. Nine haplotypes, including 4 strain-specific haplotypes, were identified by combining alleles at the CjCATH1,-2,-3, and-B1 loci. In addition, 2 and 1 amino acid substitutions (I145F, Q148H, and P245H) predicted by PROVEAN and Pol-yPhen-2 to have deleterious effects were detected in CjCATH2 and-B1, respectively. Synthetic CjCATH2 and-B1 peptides exhibited greater antibacterial activity against Escherichia coli than chicken CATH2 and-B1, respectively. Furthermore, the CjCATHB1*04 peptide exhibited less potent antimicrobial activity than other CjCATHB1 peptides examined. This is the first report of amino acid substitutions accompanied by changes in antibacterial activity in avian CATHs. These findings could be employed as indicators of improvements in innate immune response in poultry.
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