Application of methyl jasmonate and salicylic acid lead to contrasting effects on the plant's metabolome and herbivory

Phytohormone applications are used to mimic herbivory and can induce plant defences. This study investigated (i) metabolomic changes in leaf tissues of Jacobaea vulgaris and J. aquatica after methyl jasmonate (MeJA) and salicylic acid (SA) applications and (ii) the effects on a leaf-chewing, a leaf-mining and a piercing-sucking herbivore. MeJA treated leaves showed clearly different metabolomic profiles than control leaves, while the differences in metabolomic profiles between SA treated leaves and control leaves were less clear. More NMR peaks increased than decreased after MeJA treatment while this pattern was reversed after SA treatment. The leaf-chewing (Mamestra brassicae) and the leaf-mining herbivores (Liriomyza trifolii) fed less on MeJA-treated leaves compared to control and SA-treated leaves while they fed equally on the latter two. In J. aquatica but not in J. vulgaris, SA treatment reduced feeding damage by the piercing-sucking herbivore (Frankliniella occidentalis). Based on the herbivory and metabolomic data after phytohormone application, we made speculations as follows: For all three herbivore species, plants with high levels of threonine and citric acid showed less herbivory while plants with high levels of glucose showed more herbivory. Herbivory by thrips was lower on plants with high levels of alanine while it was higher on plants with high levels of 3,5-dicaffeoylquinic acid. The plant compounds that related to feeding of piercing-sucking herbivore were further verified with previous independent experiments.

Automated summary

Phytohormone applications can induce plant defenses by affecting the metabolomic profiles of leaves, leading to differences in herbivory by leaf-chewing, leaf-mining, and piercing-sucking herbivores. MeJA treatment resulted in increased NMR peaks in leaf tissues, while SA treatment showed less clear differences. High levels of threonine and citric acid in plants were associated with reduced herbivory, while high levels of glucose were linked to increased herbivory. Furthermore, specific plant compounds were found to impact feeding behavior of piercing-sucking herbivores, with implications for plant defense strategies.